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| Green, Timothy; Montagnani, Caroline; Benkendorff, Kirsten; Robinson, Nick; Speck, Peter. |
| Disease is caused by a complex interaction between the pathogen, environment, and the physiological status of the host. Determining how host ontogeny interacts with water temperature to influence the antiviral response of the Pacific oysters, Crassostrea gigas, is a major goal in understanding why juvenile Pacific oysters are dying during summer as a result of the global emergence of a new genotype of the Ostreid herpesvirus, termed OsHV-1 μvar. We measured the effect of temperature (12 vs 22 °C) on the antiviral response of adult and juvenile C. gigas injected with poly I:C. Poly I:C up-regulated the expression of numerous immune genes, including TLR, MyD88, IκB-1, Rel, IRF, MDA5, STING, SOC, PKR, Viperin and Mpeg1. At 22 °C, these immune genes showed... |
| Tipo: Text |
Palavras-chave: Crassostrea; OsHV-1; Water temperature; Antiviral response; Herpesvirus. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00162/27318/25674.pdf |
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| Green, Timothy; Montagnani, Caroline. |
| In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 mu var. Poly I:C injection induced an antiviral state in C gigas as the percentage of viral-infected oysters at 48 h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; OsHV-1; Poly I:C; Vibrio splendidus; Interferon-like response. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26293/24739.pdf |
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